Electronic cigarettes induce DNA strand breaks and cell death independently of nicotine in cell lines - Oral Oncology

Figures

Fig. 1

E-cigarette exposure increases DNA damage as measured by neutral comet assay. (A) HaCaT, UMSCC10B, and HN30 were treated with 1% by volume vaporized e-cigarette liquid with brands V2 and VaporFi, and compared to untreated controls. HaCaT cells were treated for 8 weeks, and UMSCC10B and HN30 treated for 1 week each. E-cigarette containing nicotine at 1% by volume treatment was calculated to contain 0.5 mM nicotine, so cells treated at 0.5 mM nicotine directly are shown for comparison. Results are given as mean tail length ± SEM, with at least 35 cells per sample. (B) representative images of cells captured following neutral comet assay. ∗P 

http://www.oraloncology.com/article/S1368-8375(15)00362-0/abstract